高效液相色谱法测定黄芩苷粗品中黄芩苷的含量

Determination of Baicalin in Crude Baicalin by HPLC

目的 建立黄芩苷粗品中黄芩苷的高效液相色谱法（HPLC）含量测定方法，以便控制其质量。方法 固定相用Agilent ZORBAX SB-C18柱（5μm，4．6mm×250mm），流动相为甲醇-水-冰醋酸（49：51：0．2），检测波长为275nm。结果 黄芩苷粗品中黄芩苷平均回收率为101．64％，RSD=1．75％（n=5），黄芩苷在0．102～1．020μg内峰面积与浓度线性关系良好，r=0．9997。结论 该方法简便、准确，可用于黄苓苷粗品的质量控制。

Objective To establish an HPLC method for determination of baicalin in crude baicalin. Methods Using Agilent ZORBAX SB-C18 column （ 5 μm, 4.6 mm × 250 mm）. The mobile phases consisted of methanol-water-acetic acid （49： 51 ： 0.2）. The UV detector at 275 nm. Results The average recoveries of baicalin were 101.64% and RSD were 1.75% （n = 5 ）. There is a good linearity in range of 0. 102 ～ 1. 020 μg of baicalin （ r = 0. 999 7 ）. Conclusion The method is simple, accurate and can be used to determine the contents of baicalin and crude baicalin.