白术注射液增强抗氧化酶作用的实验研究

Experimental Research on the Effct of Strengthening Anti-oxidase of Atractylis Macrocephala Injection

目的研究白术注射液对大鼠学习记忆及血、肝、肾、海马、皮质抗氧化酶SOD,GSH-PX的影响。方法采用氟哌啶醇造成大鼠获得性记忆缺失动物模型,用电跳台法观察白术注射液组、生理盐水组及模型组大鼠行为学改变,测定各组大鼠器官组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)的含量。结果与对照组比较,白术注射液使大鼠多种组织的SOD,GSH-PX显著升高,差别具有非常显著性意义(P<0.001)。结论白术注射液可升高组织的抗氧化酶含量,提示对多种疾病治疗和对人体保健作用与增强抗氧化酶作用、抵抗氧自由基损伤有关。

Objective To study on the effect of Atractylis macrocephala（AM） injection on the learning and remembering and on the expression of superoxide dismutase （SOD） and glutathione peroxidase （ GSH - PX） in serum, liver, kidney, hippocampus, cortex of aging rat. Methods Rats model of acquired remembering deficiency induced with haloperidol was emperidol was employed. Electricity - stimulated jumping stand was used for observation on rat behavior change and meanwhile concentrations of SOD and GSH - PX in different organs of the animal were detected. The changed rule and the relationship between different groups in AM animal experiment were analyzed. Results In contrast with control and groups each other, concentrations of SOD and GSH - PX strikingly increased. The differences were statistically significant （P 〈 0. 001 ）. Conclusion AM can be in up - regulating expression of anti -oxidase,suggesting that efficacy of AM in therapy of a variety of diseases and in heath prevention may be associated with enhancing of expression of anti - oxidase.