临床病原菌耐药性寡核苷酸芯片研究(Ⅰ)——耐药性基因PCR扩增及芯片制备

Study on detection of resistance in clinic pathogen by using oligonucleotide array

利用寡核苷酸芯片对临床四种常见的病原菌大肠杆菌、沙门氏菌、金黄葡萄球菌、沙眼衣原体的耐药性基因GyrA片段进行检测.结果表明不管是用荧光素TAMRA修饰的兼并引物还是用荧光素Cy3-dCTP掺入方式在PCR扩增GyrA基因的过程中对PCR产物进行荧光素标记,都可以得到足够的PCR产物用于后续的芯片杂交分析.

The anti-microbial resistance in common clinic pathogen is detected by using oligonucleotide array. The asymmetric PCR method is adopted to amplify the target gene to yield single - strand DNA target. Meanwhile, fluorescence dye is incorporated into amplicons during PCR reaction, facilitating the further microarray detection using laser scanner.