耐甲氧西林金黄色葡萄球菌SCCmec基因分型的研究

Analysis of SCCmec genotype in methicillin-resistant Staphylococcus aureus

目的了解安徽省立医院耐甲氧西林金黄色葡萄球菌(MRSA)SCCmec基因分型情况和各种基因型别的耐药特征及分子流行病学特点。方法采用VITEK32全自动微生物分析仪GPS药敏板检测MRSA菌株对14种抗菌药物的敏感性;采用PCR方法对MRSA菌株SCCmec进行基因分型。结果SCCmecⅡ型菌株4株(13.3%),SCCmecⅢ型菌株24株(80%),SCCmecⅣ型菌株2株(6.7%),未发现SCCmecI型菌株。结论SCCmecⅢ菌株为安徽省立医院存在的主要流行菌株,SCCmecⅡ型菌株和SCCmecⅢ型菌株均为多重耐药菌株。

Objective To investigate the antimicrobial resistance and molecular epidemiology profile of different methicillin-resistant Staphylococcus aureus （MRSA） SCC mec genotype in our hospital. Methods The resistance to 14 antibiotics of MRSA were determined by VITEK32 automatic microbial monitor GPS, SCC mec types were determined by PCR methods. Results Among the 30 isolates of MRSA, 4（13.3%） isolates belonged to SCC mec Ⅱ, 24 （80%） isolates belonged to SCC mec Ⅲ, 2（6.7%） isolates belonged to SCC mecⅣ were found in this test, and no isolates belonged to SCC mecⅠ was found. Conclusions SCC mec Ⅲ genotype was the major epidemic isolates in our hospital. SCC mec Ⅱ genotype and SCC mec Ⅲ genotype isolates were multi-resistant.