摘要
Heparin is a polysaccharide of glycosaminoglycan class, which consists of repeating disaccharide units of iduronic/glucuronic acid and glucosamine residues with many biological functions. Many methods have been proposed for the detection of heparin, including UV-Vis spectrophotometry, the light scattering technique, HPLC and electro phoresis and flow injection analysis, etc.. But there are few reports about the detection of heparin by means of an electrochemical method. Electroanalytical methods are useful tools in bioanalytical chemistry because of their advantages, such as their instrumental simplicity, moderate cost and portability. The binding reactions of organic molecules with biomolecules such as DNA and proteins have been widely studied. In acidic solution, heparin is highly negatively charged due to the dissociation of the sulfate and carboxyl groups in its molecule, which can easily interact with cationic dyes. Based on this principle, in this work, a new electrochemical method for the determination of heparin was developed based on the interaction of heparin with brilliant cresyl blue（BCB）.
Heparin is a polysaccharide of glycosaminoglycan class, which consists of repeating disaccharide units of iduronic/glucuronic acid and glucosamine residues with many biological functions. Many methods have been proposed for the detection of heparin, including UV-Vis spectrophotometry, the light scattering technique, HPLC and electro phoresis and flow injection analysis, etc.. But there are few reports about the detection of heparin by means of an electrochemical method. Electroanalytical methods are useful tools in bioanalytical chemistry because of their advantages, such as their instrumental simplicity, moderate cost and portability. The binding reactions of organic molecules with biomolecules such as DNA and proteins have been widely studied. In acidic solution, heparin is highly negatively charged due to the dissociation of the sulfate and carboxyl groups in its molecule, which can easily interact with cationic dyes. Based on this principle, in this work, a new electrochemical method for the determination of heparin was developed based on the interaction of heparin with brilliant cresyl blue（BCB）.
基金
SupportedbytheNationalNaturalScienceFoundationofChina(Nos.20405008,20375020),theNaturalScienceFoundationofQingdaoCity(No.042JZ114)andtheDoctoralFoundationofQUST(No.0022125).
