胰岛素受体基因多态性与2型糖尿病的相关性

Study on correlation between INSR gene polymorphism and type 2 diabetes mellitus

目的:探讨胰岛素受体基因(INSR)的第8外显子NsiⅠ多态性与湖北汉族人群2型糖尿病的相关性;方法:采用同胞对(家系内对照)和随机病例-对照两种实验设计,并结合聚合酶链反应-限制性片断长度多态性(PCR-RFLP)技术分析了224个样本的INSR基因第8外显子NsiⅠ多态性,并测定了身高、体重、腰围、臀围、血压和空腹血糖等生理指标;结果:两种实验设计中对照组与病例组的基因型和基因频率均无显著差异;结论:INSR基因在湖北汉族人群2型糖尿病的发生发展中可能不起主要作用。

Objective： To investigate the correlation between insulin receptor （INSR） gene Nsi Ⅰ polymorphism of exon 8 and type 2 diabetes mellitus in Hubei Han people. Methods： Both sib - pairs and random case - control designs were used. The genotypes in 224 samples were examined using polymerase chain reaction- restriction fragment length polymorphism （PCR- RFLP）. Height, weight, blood pressure and fasting blood glucose were measured. Results： In two experimental designs, there were no significant differences in the genotype and gene frequency between cases and controls. Conclusion： Type 2 diabetes mellitus may be little associated with INSR gene Nsi Ⅰ polymorphism of exon 8 in Hubei Han people.