摘要
目的:探讨胰岛素受体基因(INSR)的第8外显子NsiⅠ多态性与湖北汉族人群2型糖尿病的相关性;方法:采用同胞对(家系内对照)和随机病例-对照两种实验设计,并结合聚合酶链反应-限制性片断长度多态性(PCR-RFLP)技术分析了224个样本的INSR基因第8外显子NsiⅠ多态性,并测定了身高、体重、腰围、臀围、血压和空腹血糖等生理指标;结果:两种实验设计中对照组与病例组的基因型和基因频率均无显著差异;结论:INSR基因在湖北汉族人群2型糖尿病的发生发展中可能不起主要作用。
Objective: To investigate the correlation between insulin receptor (INSR) gene Nsi Ⅰ polymorphism of exon 8 and type 2 diabetes mellitus in Hubei Han people. Methods: Both sib - pairs and random case - control designs were used. The genotypes in 224 samples were examined using polymerase chain reaction- restriction fragment length polymorphism (PCR- RFLP). Height, weight, blood pressure and fasting blood glucose were measured. Results: In two experimental designs, there were no significant differences in the genotype and gene frequency between cases and controls. Conclusion: Type 2 diabetes mellitus may be little associated with INSR gene Nsi Ⅰ polymorphism of exon 8 in Hubei Han people.
出处
《现代生物医学进展》
CAS
2006年第3期5-7,共3页
Progress in Modern Biomedicine
基金
国家自然科学基金资助项目(No.30340068)
湖北省自然科学基金资助项目
关键词
胰岛素受体
基因多态性
2型糖尿病
相关性
Insulin receptor(INSR) gane
Type 2 diabetes mellitus
Polymorphism
Conelation