食管癌发生中OKT_6阳性Langerhans细胞和S－100蛋白阳性Langerhans细胞的比较观察

A Comparative Observation on the OKT_6 and S-100 Protein Positive Langerhans Cells in the Carcinogenesis of Esophageal Cancer

本研究采用ＡＢＣ免疫组化技术，对３０例食管鳞癌癌旁上皮内ＯＫＴ６阳性Ｌａｎｇｅｒｈａｎｓ细胞（ＬＣ）和Ｓ－１００蛋白阳性ＬＣ进行了标记，着重观察了ＯＫＴ６阳性ＬＣ的分布及变化规律，发现从食管正常上皮，不典型增生上皮到原位癌，随着上皮分化程度的下降，ＯＫＴ６阳性ＬＣ数目逐渐减少，形态亦发生改变，提示ＯＫＴ６阳性ＬＣ与食管癌的发生有一定的关系。另外，ＯＫＴ６阳性ＬＣ在不同病变上皮内的分布明显不同，随着上皮分化程度的下降，ＯＫＴ６阳性ＬＣ群体逐渐上移至上皮的表层，这一结果为应用细胞学的方法检测ＬＣ以判定食管上皮的分化程度提供了另一线索。同时，我们还观察到，在食管正常上皮内，ＯＫＴ６阳性ＬＣ明显多于Ｓ－１００蛋白阳性ＬＣ，而在重增及原位癌时，ＯＫＴ６阳性ＬＣ明显少于Ｓ－１００蛋白阳性ＬＣ。结果表明，在食管粘膜中，存在着不同的ＬＣ亚群，ＯＫＴ６阳性ＬＣ比Ｓ－１００蛋白阳性ＬＣ对食管内环境的变化更为敏感。

In this paper,the OKT_6 and S-100 protein positive Langerhans cells(LCs)in the ethelia adjancent to esophageal squamous cellcarcinoma from 30 patients were studied by an ABC immunohistological survey. It was found that the number of OKT6-positive LCs decreased gradually from normal ethelia to displasia to carcinoma in situ,along with the decline of the degrees of ethelial differentiation.The morphological changes of the LCs were also observed.It indicated that OKT6-positive LCs might associate to the carcinogenesis of esophagus. In addition, it was observed that the distribution of OKT6-positive LC,varied in deffernt ethelial lesion. They gradually moved into the superficial layers from the basal parts of the ethelia with the decline of ethelial differentiation. This result suggested that the investigation of LC y cytological method might be usefful in damonsrating the differential degree of esophageal ethelia-Meanwhile, we also observed that the OKT6-positive LCs, which outnumbered S-100proteion-positive LC,in normal ethelia.were much fewer than S-100 protein-positive LC, in dislpasia and carcinoma in situ. The present study shows that there exsist different subpopulations of LSs in the esophageal mucosa,and OKT6-positive LC, are more sensitive than S-100proteinpositive LCs to the change of internal enviroment of esophagus.