基因芯片在肺癌发生相关基因筛查中的应用

Expression profiling of lung cancer using cDNAmicroarray

目的研究人肺癌发生相关基因表达谱,探讨肺癌发生的分子机制。方法选取1 280个与肿瘤相关的基因克隆,制备成肿瘤相关cDNA芯片。提取人肺癌组织以及相应正常组织RNA,反转录后标记为cDNA探针,与cDNA芯片杂交,经扫描及Q uan tarray3.0软件分析后比较两种组织中的差异表达基因。结果共筛查出差异表达基因52条。其中表达上调基因35条,下调基因17条;按照基因功能可分为运输载体、代谢相关基因、细胞信号转导分子、细胞骨架、转录调控因子以及未知功能基因。结论基因芯片可用于肺癌相关基因表达谱的筛查,为明确肺癌发生机制提供重要参考。

Objective： To investigate the gene expression profile of human lung cancer. Methods： 1280 cancer-related genes were selected from human cDNA library and spotted to the silanated slides. Total RNA from samples were extracted and labeled with fluorescent dye. The labeled probes were hybridized to the microarray and scanned with ScanArray4000 and analyzed by Quantarray3. 0. Results： 52 genes associated with lung carcinogenesis were found, which were grouped into transporter, adhesion molecules, cytoskeleton, transcription regulator, genes associated with metabolism, immune response, and EST according to functions. 35 genes were positive to lung carcinogenesis and 17 genes were negative to lung carcinogenesis. Conclusion ： The screen of gene expression profile of human lung cancer the can provide valuable information for the research of molecular mechanism of the lung carcinogenesis.