心肌细胞培养液对鼻咽癌细胞生长的体外抑制作用实验研究

Experimental Research on Inhibitory Action of Cardiac Muscle Conditioned Medium on Growth of Nasopharyngeal Carcinoma Cell

目的:恶性肿瘤可转移至机体所有器官,但在心肌中罕见。本实验拟通过研究心肌微环境因素对不同转移潜能的鼻咽癌细胞的影响,探讨心肌内转移瘤罕见的机制。方法:原代培养新生Wistar大鼠心肌细胞,用 MTT法分析和比较心肌细胞条件性上清液(CMCM)对高转移潜能鼻咽癌细胞的体外抑制作用。研究设空白对照, 以顺铂作为CMCM的阳性对照,以正常细胞鼠肾小球系膜细胞为阴性对照。结果:鼻咽癌细胞与不同浓度的CM CM共同培养后,细胞存活率明显下降,与DMEM相比差异均具有显著性(P<0．05),而对正常细胞的细胞存活率无明显影响(P>0．05)。DDP对鼻咽癌细胞和正常细胞均具有显著性抑制作用(P<0．05)。结论:新生大鼠心肌细胞上清液可选择性的抑制鼻咽癌细胞的增殖;这种抑制作用与化疗药物顺铂作用机制可能不同。心肌微环境中可能存在某种抑瘤物质,这可能是心肌转移瘤罕见性的主要机制。

Objective： Malignant tumors spread and metastasize in the majority of the organs, but very rare in cardiac muscle. This study was conducted to explore the effect of organic microenvironment of cardiac muscles on the nasopharyngeal carcinoma cell with high metastasis potential, and to investigate the mechanism of the rarity of metastasis in cardiac muscles. Methods： Primary culture of newborn Wistar rat cardiac muscle cells was established, and the murine cardiac muscle conditioned medium（CMCM） was prepared to test its effect in vitro on nasopharyngeal carcinoma cells by MTr assay. Cisplatin was used as positive control for CMCM, DMEM was used as negative control. Murine benign renal cells-mesangial cells were used as negative control for nasopharyngeal carcinoma cells. Results： Proliferation of tumor cell lines of CNE-2 was significantly restrained when cultured with CMCM, while the murine benign renal cells were not affected（ P 〈 0. 05 ）. Conclusion： Cardiac muscle cells could selectively iahibite the proliferation of nasopharyngeal carcinoma cells in vitro while benign cells are not affected.