摘要
目的:研究青蒿琥酯的抗肿瘤作用。方法:用青蒿琥酯或全铁转铁蛋白联合青蒿琥酯进行体外抗肿瘤实验,用MTT法检测青蒿琥酯对体外培养人结肠癌HCT-8细胞、人红白血病K562细胞及人乳腺癌MCF-7细胞的杀伤作用。结果:体外实验表明青蒿琥酯对上述3种人肿瘤细胞均有杀伤作用,对HCT-8、K562、MCF-7的 IC50值为1.99μg/ml、1.62μg/ml、10.54μg/ml;加用1mg/ml全铁转铁蛋白处理3小时后再给予青蒿琥酯,其IC50 值分别为3.54μg/ml、4.14μg/ml、11.95μg/ml。结论:青蒿琥酯钠在体外对HCT-8、K562、MCF-7等细胞有明显的杀伤作用,加用全铁转铁蛋白未明显增强对肿瘤细胞的杀伤作用。
Objective: To inverstigate the antitumor efficacy of artesunate. Methods: To detect the cytotoxicity of artesuhate on HCT-8 (human colo cancer cell llne), K562 (human blood cancer ceil llne) and MCF-7 (human breast cancer cel llne) by using MTT assay. Results: Artesunate had antitumor activity in vitro, the 50% inhibiting concentrotions (IC50) of artesunate to HCT-8, K562 and MCF-7 were 1. 99μg/ml,1. 62μg/ml and 10. 541μg/ml respectively. With preincubation of holo-transferrin lmg/ml for 3 hours before administration of artesurate, the ICS0 were changed to 3. 54μg/ml, 4. 14μg/ml and 11. 95μg/ml respectively. Conclusion: The experiment shows that artesunate has antitumor activity in vitro. Combination with holo-transferrin can not farther enharce its antitumor activity.
出处
《四川肿瘤防治》
2006年第2期89-91,94,共4页
Sichuan Journal of Cancer Control