摘要
目的探讨CIK过继免疫治疗对手术后肺癌患者T细胞及其活化抗原表达的影响。方法采用流式细胞术检测40例肺癌患者外周血T细胞表型CD3、CD4、CD8和及其活化抗原标志CD69、CD25、HLA-DR。结果肺癌患者CD3+、CD4+、CD4/CD8比值明显低于对照组并呈显著性差异,而CD8+细胞高于对照组(P<0.01);肺癌患者CD69、CD3+/HLA-DR+、CD4+/CD25+与对照组比较无显著性差异。经CIK治疗后CD3+、CD4+、CD4/CD8比值都升高,其中CD4+细胞与治疗前比较呈显著性差异(P<0.05),而CD8+细胞与治疗前比较降低(P<0.05);CD69、CD3+/HLA-DR+均升高(P<0.05),CD4+/CD25+在治疗后有增高趋势,但无显著性差异。结论CIK过继免疫治疗对肺癌患者免疫功能的恢复具有促进作用。
Objective To investigate the T cell immunophenotype and activation antigen expression in patients with lung cancer treated with CIK.Methods The T cell subsets and CD69,HLA-DR, CD25 on peripheral blood from patients with lung cancer( n = 40)were detected with flow cytometry.Results The CD3^+ , CD4^+ and CD4/CD8 from pre-operation patients with lung cancer are significantly lower than that of the control( P 〈 0.01 ). Comparied with the control group, the CD8^+ cells from patients with lung cancer were increased( P 〈 0.01). The CD3^+ , CD4^+ and CD4/CD8 from post-operation patients treated with CIK were increased. Comparied with pre-operation, the difference of CD4^+cells is significant( P 〈 0.05) and the CD8^+ ceils were docreased( P 〈 0.05), CD69,CD3^+/HLA - DR ^+ were increased ( P 〈 0.05 ). Conclusion The immunological function of patients with lung cancer were decreased and could be enhanced by CIK adoptive immunotheropy.
出处
《中国实验诊断学》
2006年第5期455-457,共3页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技厅科研项目(200564)
吉林省科技厅重点科研项目(20040414)
吉林省科技厅重点项目(编号:20040401-5)
吉林省科技厅国际合作项目(编号:20040707-2)
2004年长春市科技局计划项目(编号:2004218)