过继免疫治疗对手术后肺癌患者T细胞及其活化抗原表达影响的研究

Effects of adoptive immunotheropy on the T cell immunophenotype and activation antigen expression in patients with lung cancer

目的探讨CIK过继免疫治疗对手术后肺癌患者T细胞及其活化抗原表达的影响。方法采用流式细胞术检测40例肺癌患者外周血T细胞表型CD3、CD4、CD8和及其活化抗原标志CD69、CD25、HLA-DR。结果肺癌患者CD3+、CD4+、CD4/CD8比值明显低于对照组并呈显著性差异,而CD8+细胞高于对照组(P<0.01);肺癌患者CD69、CD3+/HLA-DR+、CD4+/CD25+与对照组比较无显著性差异。经CIK治疗后CD3+、CD4+、CD4/CD8比值都升高,其中CD4+细胞与治疗前比较呈显著性差异(P<0.05),而CD8+细胞与治疗前比较降低(P<0.05);CD69、CD3+/HLA-DR+均升高(P<0.05),CD4+/CD25+在治疗后有增高趋势,但无显著性差异。结论CIK过继免疫治疗对肺癌患者免疫功能的恢复具有促进作用。

Objective To investigate the T cell immunophenotype and activation antigen expression in patients with lung cancer treated with CIK.Methods The T cell subsets and CD69,HLA-DR, CD25 on peripheral blood from patients with lung cancer（ n = 40）were detected with flow cytometry.Results The CD3^＋ , CD4^＋ and CD4/CD8 from pre-operation patients with lung cancer are significantly lower than that of the control（ P 〈 0.01 ）. Comparied with the control group, the CD8^＋ cells from patients with lung cancer were increased（ P 〈 0.01）. The CD3^＋ , CD4^＋ and CD4/CD8 from post-operation patients treated with CIK were increased. Comparied with pre-operation, the difference of CD4^＋cells is significant（ P 〈 0.05） and the CD8^＋ ceils were docreased（ P 〈 0.05）, CD69,CD3^＋/HLA - DR ^＋ were increased （ P 〈 0.05 ）. Conclusion The immunological function of patients with lung cancer were decreased and could be enhanced by CIK adoptive immunotheropy.