中国HCV/HIV合并感染者T淋巴细胞表面CD81、CXCR3表达的研究

Study on surface expression of CD81 and CXCR3 on T lymphocytes from patients with HCV/HIV co-infection in China

目的探讨T淋巴细胞表面受体CD81、CXCR3,在丙肝病毒(HCV)单纯感染,艾滋病病毒(HIV)单纯感染和HCV/HIV合并感染过程中的表达及意义。方法采用流式细胞术,检测HCV感染组(n=21),HIV感染组(n=14),HCV/HIV感染组(n=28)及正常对照组(n=30)人外周血CD4+T淋巴细胞和CD8+T淋巴细胞表面CD81、CXCR3的表达。结果HIV感染组及HCV/HIV合并感染组CD4+T细胞表面CD81、CXCR3表达显著降低(P<0.001),CD8+T细胞表面CD81、CXCR3表达显著升高(P<0.001);HCV感染组CD4+及CD8+T细胞表面CXCR3表达轻度升高但差异不显著,CD81在CD4+T细胞表面轻度升高而在CD8+T细胞表面明显升高(P<0.01)。结论中国HCV/HIV合并感染患者外周血T淋巴细胞表面受体CD81、CXCR3,在CD4+T细胞表面表达降低,而在CD8+T细胞表面表达升高。

Objective To investigate expression and significance of CD81, CXCR3 in HCV mono-infection, HIV mono-infection and HCV/IUV co-infection. Methods We detected the surface expression of CD81 and CXCR3 on CD4^＋ T cells and CD8^＋ T cells from PBMC isolated from HCV infection（ n = 21 ）, HIV infection（ n = 14）, HCV/HIV co-infection（n = 28） and normal control（ n = 30） by flow cytometry. Results The expression of CD81 and CXCR3 on CD4^＋ T cells decreased significandy（ P 〈 0.001）, whereas it significandy increased on CD8 ^＋ T cells in HIV infection and HCV/HIV co-infection group（ P 〈 0.001 ）. In HCV infection group, the expression of CXCR3 on CD4^＋T cells as well as CD8^＋T ceils increased but not significantly, and expression of CD81 on CD4^＋ T ceiLs slightly increased but significantly increased on CD8^＋ T ceiLs（ P 〈 0.01 ）. Conclusion The surface expression of CD81 and CXCR3 on CD4^＋ T lymphocytes decreased, whereas it increased on CD8^＋ T lymphocytes from patients with HCV/HIV co-infection in China.