牛白细胞粘附缺陷病(BLAD)检测方法的建立

The Construction of The Tested Method for Bovine Leukocyte Adhesion Deficiency (BLAD)

为了准确诊断牛白细胞粘附缺陷病(bovine leukocyte adhesion deficiency,BLAD),根据BLAD病因,建立RT-PCR(reverse transcriptase PCR)、再进行TaqⅠ酶切扩增产物作为本病检测技术。临床应用表明该方法具有简便、快速、特异性好等优点,所以,该方法建立不仅为BLAD检测带来了方便,同时也给人和其他动物疾病的诊断提供了有利参考。

In order to correctly diagnose bovine leukocyte adhesion deficiency （BLAD）, according to the pathogeny of BLAD, the means of amplifying a CD18 gene fragment via reverse transcriptase-PCR followed by restriction digestion with TaqⅠ were constructed and regarded as the technology of diagnosed BLAD. The clinic practice showed that the means had the simple, quick and special advantage. So the means not only offered to convenience for BLAD diagnosis, but also brought favourable reference for disease of human and other animal.