发酵猪血优良菌株A32发酵条件的研究

Study on Swine Blood Fermentation Conditions of Bacillus A32

从农村个体屠宰场土壤中分离到发酵猪血优良菌株A32,为了进一步提高A32菌株发酵猪血的能力,通过单因素试验以及正交试验确定了A32最优培养基配方:豆粕粉2%、玉米粉3%、0.4mmol/LMn2+、Na2HPO4·12H2O0.4%、KH2PO40.03%、Na2CO30.01%、pH7.2;同时还研究了培养时间、摇瓶转速、接种量、温度对产酶的影响,试验表明培养时间48h,接种量3%、温度30℃组合有利于蛋白酶的产生,摇瓶转速190r/min较为理想。

Bacillus A32 has been isolated from the slaughtering house soil whose ability on fermenting swine blood was superior. To improve the ability on fermenting swine blood by A32, fermentation conditions were studied on various factors and orthogonal experiments. The optimum fermentation medium containing soybean powder 2%, cornmeal 3%, Na2HPO4 · 12H2O 0.4%, KH2PO4 0.03%, Na2CO3 0.01% and Mn^2＋ 0.4mmol/L with pH7.2. At the same time, the effects for producing protease such as time, rotation speed, inoculum size, temperature and metal ions were studied. The results showed that 48h, 190r/min, 2% inoculum size and 30℃ are optimum for producing protease.