阿魏菇多糖的抗氧化功能及其对果蝇寿命的影响

Antioxidation Activity of Pleurotus nebrodensis Mycelium Polysaccharides in Mice and Its Effect on Senescence-resistance of Fruit Flies

目的:研究了阿魏菇多糖抗氧化延缓衰老作用。方法:按多糖剂量不同,将试验动物分组;分别进行果蝇生存实验和小鼠抗氧化实验。测定指标包括:果蝇组死亡率,小鼠组的丙二醛、谷胱甘肽过氧化物酶、超氧化物岐化物等的含量或活性。结果:发现果蝇在较高和高剂量组中平均寿命和最高寿命都显著高于对照(p<0.05);在对老年小鼠作用的实验中,阿魏菇多糖(PNMP)各剂量组小鼠红细胞SOD活力均高于老龄对照组,其中,中剂量组明显升高(p<0.05)。各阿魏菇多糖组血清中MDA含量低于老年对照组,其中中剂量组显著降低(p<0.05)。各剂量组脑中的MDA、LPF均有不同程度的下降,PNMP对H2O2诱导小鼠红细胞溶血有明显抑制作用。结论:阿魏菇多糖具有明显的抗氧化延缓衰老作用。

Objective： To investigate the effect of Pleurotus nebrodensis mycelium polysaccharides （PNMP） against oxidation and senescence. Method： According to different polysaccharide doses, grouped animals were respectively conducted in experiments of fruit fly survival and mice ＇ s antioxidation capability. The assay indexes were： mortality of fruit fly group, and MDA content, activity of GSH-PX and SOD etc in mice. Results： regon K＇s average-life-span and max-life-span after receiving PNMP were both significantly higher, compared with the control group（p〈0.05）.When mice were fed by PNMP, SOD activity in their RBC between different dose groups all significantly increased by the control, and the value was very obvious in middle dose group （p〈0.05）. Compared with the control group, MDA, LPF contents in serum and brain organs between different dose groups had declined, and the middle dose group kept the sharp trend （p 〈 0.05） in terms of MDA in serum. In addition, PNMP also showed obvious inhibition effect on H2O2-induced rats＇ RBC hemolysis. Conclusion： PNMP had obvious part of antioxidation and senescence-resistance.