不同方法处理同种异体肋软骨的组织学改变

Histological changes in the homograft costal cartilage after different modes of processing

目的采用不同方法对家兔肋软骨进行处理,观察移植后的组织学改变,旨在比较几种方法的优劣。方法用60Co照射、酒精浸泡、冷冻等3种方法处理的同种肋软骨对40只家兔进行移植,12周后取软骨连续组织切片,光镜下观察并进行综合评价。结果组织学反应以炎细胞浸润、坏死、血管反应及纤维组织增生为主。经统计学处理,3种方法在坏死及炎细胞浸润两种组织学改变中的差异有显著意义(P<0.05)。结论①本组实验60Co照射组软骨坏死的阳性率最低;②机体对同种异体肋软骨产生不同程度的排斥反应,且个体差异较大,与处理方法有关;③移植的同种异体肋软骨中伴纤维组织增生占50%左右,提示移植肋软骨在体内可能被宿主的纤维结缔组织所代替,可以维持形态。

OBJECTIVE To compare different methods of processing of homograft costal cartilage. METHODS Homograft costal cartilage samples were processesd by three methods-irradiation, freezing, and alcohol fixation-before being inserted into the rabbit nasal dorsum. They were taken out 12 weeks later and evaluated for histological responses under a optical microscope. RESULTS The histological responses consisted of inflammatory cell infiltration, necrosis, vasal responses and fibroplasia. The was a significant different in degree of inflammatory cell infiltration and necrosis in all three groups（P〈 0.05）.CONCLUSION ①lradiation was the best method of the three. ② The histological response depended on the method of processing. ③The homogreft costal cartilage was likely to be replaced by fibroplasia, though the nasal shape would be hold out.