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弱阴离子交换-超滤两级纯化浓缩真鲷卵黄蛋白原

PURIFICATION AND CONCENTRATION OF VITELLOGENIN FROM PAGROSOMUS BY ANION EXCHANGE LIQUID CHROMATOGRAPHY AND ULTRAFILTRATION
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摘要 利用弱阴离子交换色谱,从雌激素17β-雌二醇诱导的真鲷鱼血清中分离纯化出卵黄蛋白原粗组分,以超滤法进一步除去杂质蛋白,并脱盐浓缩,获得纯度较高的卵黄蛋白原(Vtg),两级纯化得到的Vtg经常规聚丙烯酰胺凝胶电泳(Native-PAGE)分析,证实真鲷鱼Vtg有三种形式,分子量分别为665kDa,635kDa和355kDa;变性聚丙烯酰胺凝胶电泳(SDS-PAGE)分析结果表明,它们由185kDa左右的蛋白亚基组成. With uhrafiltration and anion exchange liquid chromatography, a two-step method had been developed for purification of vitellogenin （Vtg） from the serum of Pagromus major induced by 17β-estradiol （ E2 ） . The results showed that most impurities could be removed during the first step by weak anion exchange liquid chromatography and further clean-up through ultrafihration. In Native-PAGE, the Vtg purified by the two steps showed three forms, with molecular weights approximately 665kDa, 635kDa and 355kDa, respectively. The result of SDS-PAGE indicated that the molecular weight of Vtg monomers was about 185 kDa. High resolution anion exchange high performance liquid chromatography was applied to analyze the Vtg purified from the twostep method, and approved the high purity. The purified Vtg could be used for further analysis.
作者 刘宝敏 袁东星 吴翠琴
机构地区 近海海洋环境科学国家重点实验室厦门大学环境科学研究中心
出处 《环境化学》 CAS CSCD 北大核心 2006年第3期306-309,共4页 Environmental Chemistry
基金 国家自然科学基金(20277030)资助项目
关键词 弱阴离子交换色谱 超滤 卵黄蛋白原 weak anion exchange liquid chromatography, ultrafihration, vitellogenin.
分类号 Q593-33 [生物学—生物化学]
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参考文献16

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二级参考文献13

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环境化学
2006年 第3期

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