光倒刺鲃与中华倒刺鲃染色体组型研究

Karyotype Analyses of Spinibarbus hollandi Oshima and S.sinensis Bleeker

采用植物血球凝集素(PHA)体内注射,肾组织细胞体内短期培养,空气干燥法制备了光倒刺鲃、中华倒刺鲃的染色体。并对其组型进行分析研究。结果表明:光倒刺鲃的染色体组型公式是2n=100=18m+30 sm+26 st+26 t,NF=148;中华倒刺鲃的染色体组型公式是2n=100=18m+28 sm+28 st+26 t,NF=146。

The chromosome specimens of Spinibarbus hollandi and S. siensis were prepared from short-term in vitro culture of the kidney cells-PHA by Mr-drying technique and their karyotypes were analyzed. The result showed that there were 100 chromosomes in diploid of the fish. The karyotype formula of chromosome from Spinibarbus hollandi and S. siensis was 2n = 100 = 18m ＋30sm ＋.26st ＋.26t, NF = 148, and 2n = 100 = 18m ＋28sm ＋28st ＋26t, NF = 146, respectively.