间接ELISA测定血清中RI含量方法的建立

Establishment of indirect ELISA technique to test the expression of ribonuclease inhibitor in blood serum

目的:建立检测血清中核糖核酸酶抑制因子(Ribonuclease Inhibitor,RI)含量的间接酶联免疫吸附法(ELISA),并分析该方法的特异性和敏感性。方法:用血清样品包被酶标板,兔抗人RI抗体为一抗,辣根过氧化物酶标记的羊抗兔抗体为二抗,建立血清RI定量检测的间接ELISA法。结果:所建立的用于血清中RI含量检测的间接ELISA方法特异性好,灵敏度高。结论:间接ELISA法可以用于测定血清中的RI抗原含量,具有良好的灵敏度和稳定性,能够有效的反映出人血清中的RI表达情况。

Objective：To establish an indirect ELISA technique for the detection of the content of Ribonuclease Inhibitor （RI） antigen in serum and analyse the specificity,sensitivity and reliability of the method.Methods：The RI in serum was used as antigen and rabbit anti-RI was used as the first antibody and goat anti-rabbit IgG-HRP was used as the second antibody. The best dilution for rabbit anti-RI and goat anti-rabbit IgG-HRP were decided according to the best reaction condition standards for ELISA,the indirect ELISA for the detection of RI antigen expressed in serum was established.Results：The indirect ELISA is stable,highly sensitive and specific.Conclusion：The indirect ELISA is a specific and sensitive method in the detection of the expression of RI antigen in serum.