藻类分子生物技术两年评──基因工程及其上游──分子遗传学

TWO-YEAR REVIEW ON ALGAL MOLECULAR BIOTECHNOLOGY──MOLECULAR GENETICS AND GENETIC ENGINEERING

根据1992年1月-1994年6月国内外发表资料，评述藻类分子遗传学和基因工程领域的最新进展。认为：两年来藻类分子遗传学发展迅速，经济藻类基因工程研究已经有所突破。藻类学研究正在进入分子时代，分子生物技术成为推动藻类学研究和藻类资源开发的新动力。

Development of algal molecular genetics and genetic engineering studiesfrom Jan., 1992 to June, 1994 is reviewed in this paper. Researches in algal molecular genetics developed rapidly in the past two years. Genetic engineering ofeconomically valuable algae is also progressing very fast. The results of this studyon plasmids from Spirulina platensis and the agarophyte Gracilaria asiatica,studied by the authors, will be used to construct vectors for genetic engineering. Biolistics was proved by us as an effective method to introduce foreigngenes into kelp cells where their stable expression was observed. We also foundthat kelp was very sensitive to chloramphenicol, and that CAT gene was a usefulselective marker. The work is still progressing. Both fundamental phycology studyand algal resource exploitation are being advanced to a new stage by algal molecular biotechnology.