HPLC同时测定心可舒胶囊中葛根素、丹参酮ⅡA含量

Simultaneous determination of puerarin and tanshinone Ⅱ A in Xinkeshu Capsule by HPLC

目的：测定心可舒胶囊（葛根，丹参等）含量。方法：采用HPLC法C18柱，流动相：甲醇-水（85：15），流速：1．0mL／L，波长：250nm。结果：葛根素和丹参酮ⅡA进样量分别在21．00—63．00μg和20．02～60．06μg范围内线性关系良好，r值分别为0．9998和0．9997，平均加样回收率分别为97．7％和97．0％。结论：方法操作简便、易行，可有效地控制制剂的质量。

AIM ： To determine puerarin and tanshinone ⅡA in Xinkeshu Capsule （ Radix Puerariae Lobatae, Radix et Rhizoma Salviae Mihiorrhizae） by HPLC simultaneously. METHODS： HPLC C18 column was used with methanol-water（85：15） as the mobile phase. The flow rate was 1.0 mL/min, and the wave length was at 250 nm. RESULTS ： There were good liner relationships for puerarin and tanshinone ⅡA with the sample input quantity at the range of 21-63μg （ r = 0. 999 8 ） and 20.02-60.06μg（ r = 0.999 7 ） , respectively. The average recoveries of puerarin and tanshinone ⅡA were 97.7% and 97.0%, respectively. CONCLUSION ： This method is simple, accurate, effective and suitable for the quality control of Xinkeshu Capsules.