青春双歧杆菌细胞壁免疫调节和抑瘤作用研究

Study on Immunomodulation and Tumor-inhibition of Bifidobacterium Cell Wall in Mice.

目的探讨青春双歧杆菌细胞壁对小鼠腹腔巨噬细胞功能的影响及其对人移行细胞膀胱癌细胞的体外抑制作用。方法采用青春双歧杆菌细胞壁腹腔注射于KM小鼠,以ELISA法测定腹腔巨噬细胞产生的细胞因子,如IL-1、IL-12及TNF-α的含量,采用MTT法测定双歧杆菌细胞壁体外对人移行细胞膀胱癌细胞的抑制作用。结果青春双歧杆菌细胞壁注射组腹腔巨噬细胞产生的IL-1、IL-12及TNF-α的含量分别明显高于对照组,两者比较具有显著差异;不同浓度双歧杆菌细胞壁在体外均能抑制T24细胞生长,且浓度越大,对该细胞的抑制作用越强。结论青春双歧杆菌细胞壁成分能激活小鼠腹腔巨噬细胞,使之分泌多量的IL-1、IL-12及TNF-α,并且在体外能抑制人移行细胞膀胱癌细胞的生长。

Objective To explore the immunologic regulation of the bifidobacterium adolescence cell wall （BCW） on function of macrophage in mice and tumor inhibition of BCW on human transitional cell carcinoma of bladder （BTCC） outside of body.Methods BCW were injected into abdomen of KM mice, the level of IL - 1, ILl2 and TNF - α which produced by macwphage was determined by ELISA reagent box. The role of tumor inhibition was determined by MTT method. Results The level of IL - 1, IL-12 and TNF - α of BCW injection group was higher than of control group （ P 〈 0.01 ） ; The tumor - inhibition become stronger with the increase of BCW concentration. Conclusion BCW can activate macmphage to secrete more IL- 1, IL-12 and TNF- α; BCW can inhibite the growth of BTCC outside body.