蝴蝶槐的组织培养和快速繁殖技术

Studies on Rapid in Vitro Propagation of Sophora japonica var.oligophylla

以蝴蝶槐的幼叶和茎尖为试材进行离体组培快繁的研究结果表明:幼叶适合于作为繁殖材料,最佳诱导产生不定芽的培养基为MS+6-BA1.0mg/L+NAA0.2mg/L,增殖培养基为MS+6-BA2.0mg/L+NAA0.2mg/L,生根培养基1/2MS+IBA0.2mg/L+ABT1.0mg/L。试管苗经适宜条件炼苗驯化后移植大田,成活率可达85%以上,而且生长良好。

Rapid in vitro culture with shoot tips and leaves of Sophora japonica var. oligophylla was carried out. The results showed that leaves were adapted to as propagation material, the optimum medium for adventitious bud induction was MS ＋ 6- BA1.0mg/L ＋ NAA0.2mg/L, the multiplication medium was MS ＋ 6 - BA2.5mg/L ＋ NAA0. 1mg/L, and the root - promoting medium was 1/2MS ＋ IBA0.2mg/L ＋ ABT1.0mg/L. The survival rate was up to 85 % after the shoots in vitro domesticated under optimum conditions, and the plants grew well.