猪肝细胞在聚砜膜生物界面生长的初步研究

Preliminary study of porcine hepatocytes cultured on organism interface of polysulfone membrane

目的初步了解中国实验乳猪肝细胞在聚砜膜生物界面生长的情况,探索聚砜膜作为生物人工肝中空纤维反应器膜材料的可行性。方法将分离的新生实验小型猪肝细胞接种到聚砜膜和聚苯乙烯界面,进行形态学观察及生物学功能测定,观察培养猪肝细胞的酶漏出量。结果乳猪肝细胞在聚砜膜界面培养呈球型黏附、铺展,形成多细胞聚集体。与聚苯乙烯组相比较,聚砜膜组的尿素合成和蛋白分泌能力更强,氨转化率也明显提高,LDH和AST的漏出量无显著差异。结论聚砜膜是良好的乳猪肝细胞生长的生物界面,可以作为生物人工肝中空纤维反应器的膜材料。

Objective To preliminarily understand the situations of porcine hepatocytes cultured on the organism interface of polysulfone membrane and explore the feasibility of polysulfone membrane as bioartificial liver hollow fibers bioreactor＇s membrane material. Methods The hepatocytes isolated from experimental suckling minipigs were seeded onto the surfaces of polysulfone membrane and polystyrene. Cellular morphology was observed and both the biological function and enzyme release of porcine hepatocytes were examined. Results Porcine hepatocytes attached and spread on polysulfone membrane surface and formed spheroidal aggregates. As compared with hepatocytes cultured on polystyrene surface, the ability of urea synthesis and albumin secretion was increased significantly and the rate of ammonia elimination was raised significantly on polysulfone membrane surface. There was no significant difference in enzyme release of the hepatocytes cultured on the surfaces of polysulfone membrane and polystyrene. Conclusion Polysulfone membrane is a kind of fine organism interfaces and could be used as inartificial liver hollow fibers bioreactor＇s membrane material.