大鼠无肝状态肾脏罗库溴铵相关转运蛋白OATP-3及其基因的表达变化的研究

Expression variation of kidney OATP-3 relating to rocuronium metabolism in rats during anhepatic phase

目的研究无肝状态前后大鼠肾脏罗库溴铵相关转运蛋白阴离子转运多肽3(organanion transportingpoly peptides3,oatp3)及其基因表达的变化,以期初步阐释罗库溴铵无肝期肝外代谢特点形成的原因。方法12只大鼠随机分为2组(6只/组),对照组与阻断肝门60min组,取各组大鼠肾脏组织,采用Westernblot检测各组OATP3蛋白的表达。采用逆转录聚合酶链式反应(RT PCR)检测各组oatp3mRNA的表达。结果大鼠肾脏组织OATP3蛋白表达水平阻断肝门60min组表达水平高于对照组(P<0.01);大鼠肾脏组织oatp3mRNA表达水平阻断肝门60min组明显高于对照组(P<0.05)。结论大鼠肾脏组织oatp3在无肝期比无肝期前增多,oatp3基因表达在无肝期比无肝期前增多,这可能是罗库溴铵无肝期肝外代谢增强的机制。

Objective To investigate the expression variation of kidney OATP-3 in rats relating to rocuronium metabolism in anhepatic phase and to primarily explain the reason of extrabepatic metabolism characters of rocuronium. Methods Twelve rats were distributed to 2 groups randomly with 6 in each： Group A （control group） and Group B in which the hepatic portal devascularization was performed for 60 min. Kidney tissues of the rats were taken, oatp-3 mRNA was detected by RT-PCR and OATP-3 protein by Western blotting. Results The expression levels of kidney OATP-3 mRNA and protein in Group B were significantly higher than those Group A （P 〈 0. 01 ）. Conclusion Kidney OATP-3 and its gene expression levels are increased in rats during anhepatic phase than before hepatic portal dissociation, which is presumably due to the enhanced extrabepatic metabolism of rocuronium in anhepatic phase.