定量分析人脑星形细胞肿瘤组织芯片中血管生成与肿瘤微血管结构

Quantitative analysis of angiogenesis and tumor microvascular architecture in microarray of human astrocytoma

目的定量检测人脑星形细胞肿瘤组织芯片中血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达及微血管结构参数及其意义。方法采用组织微阵列技术构建包含正常人脑组织、弥漫型星形细胞瘤(WHOⅡ级)、间变型星形细胞瘤(WHOⅢ级)、胶质母细胞瘤(WHOⅣ级)及阳性对照的168点阵的石蜡组织芯片。用免疫组织化学双重染色法标记组织芯片中的VEGF及CD34的表达。采用图像分析软件对组织芯片上VEGF的表达强度以及微血管结构参数(包括血管数、血管平均周长)进行定量测试。结果星形细胞肿瘤各级别之间除VEGF阳性单位(positiveu nit,PU)值、血管平均周长在Ⅲ级与Ⅳ级之间没有差异外,其他级别之间的差异都有显著性差异(P<0.05)。结论星形细胞肿瘤中VEGFPU值与微血管结构参数可作为星形细胞肿瘤分级和血管生成程度的重要指标。

Objective To observe the parameters of the microvascular architecture expression of vascular endothelial growth factor （VEGF） and the in human astrocytomas. Methods A tissue microarray with 168 points including diffuse astrocytoma （ WHO grade Ⅱ ）, anaplastic astrocytoma （ WHO grade Ⅲ ） , glioblastoma （WHO grade Ⅳ ） and control tissues was constructed. The expression of VEGF and CD34 was detected by double-labeling immunohistochemistry on tissue microarray. The expression intensity of VEGF and microvascular architecture parameters including vessel number and vessel mean perimeter were analyzed with Image Pro Plus5.0. Results The positive unit （PU） of VEGF has a positive correlation with vessel mean perimeter in diffuse astrocytoma, anaplastic astrocytoma and glioblastoma. Significant differences （P 〈 0. 05 ） were found in each grade of astrocytoma except PU and vessel mean perimeter between anaplastic astrocytoma and glioblastoma. Conclusion VEGF PU and microvascular architecture parameters can be used as an important indicator for assessing the grade and angiogenesis extent of human astrocytomas