非小细胞肺癌中survivin蛋白的表达及其与p53表达的关系

Expression of survivin Protein and Its Relationship with Expression of p53 Protein in Non-Small Cell Lung Carcinoma

目的探讨非小细胞肺癌组织中survivin蛋白的表达与临床病理特征的关系及其与p53表达的相关性。方法采用免疫组化的链霉菌抗生物素过氧化酶法(Streptavidin-Peroxidase,SP)检测80例NSCLC组织和20例正常肺组织中survivin、p53蛋白表达情况。结果80例非小细胞肺癌组织中sur-vivin蛋白阳性例数49例,表达率为61.3%,在肺良性病变组织中,未阳性表达,两组比较差异有显著性(P<0.05);在III^IV期肺癌组织中survivin蛋白阳性表达率显著高于I^II期肺癌组织(P<0.05),而与组织学分型、分化程度、淋巴结转移与否无关(P>0.05);survivin、突变型p53蛋白在肺癌组织中的表达呈正相关。结论survivin蛋白表达与肺癌的TNM分期密切相关,有可能成为判断病情和评价预后的指标,survivin有望成为肺癌诊断的肿瘤标志物及基因治疗的新靶点;survivin、突变型p53蛋白在肺癌组织的协同高表达可能促使肺癌病情的恶性发展。

Objective To study the expression of survivin and its relationship with the clinical pathological features of the NSCLC（non-small cell lung carcinoma）. Methods The expression of survivin,p53 protein was detected in 80 eases of NSCLC tissues and 20 cases of normal lung tissue by immunohistochemical Streptavidin-Peroxidase （SP） method. Results survivin was expressed in 49 of 80（61.3%）cases of lung carcinoma and in 0 of 20（0） cases of normal lung tissues with the expression of survivin in lung carcinoma significantly higher than that in normal lung tissues（P〈0. 05）. The positive rate of survivin in stage Ⅲ- Ⅳwas significantly higher than that of survivin in stage Ⅰ-Ⅱ （P〈0. 05）;There was no relationship between survivin gene expression and histological subtypes, differentiation, TNM stages, lymph node metastasis （P〉0. 05）,There is the positive correlation existing in the expression of Survivin,mutation-P53 in lung carcinoma. Conclusion survivin with only expression in the cancer tissues, not in benign lung tissues,may play an important role in lung carcinogenesis and be identified as a definite diagnostic marker and a potential therapeutic target in NSCLC. Cooperative high expression of survivin and mutation-P53 in lung cancer tissues may make lung cancer develop quickly.