大鼠肝再生过程中肝细胞caspase-3和caspase-8活性的变化

Changes of hepatic caspase-3 and caspase-8 activities during liver regeneration in rats

目的:研究大鼠肝再生过程中肝细胞caspase-3和caspase-8活性的变化规律。方法:雄性SD大鼠70%肝切除(PH),制作肝再生模型,制备肝匀浆液,经离心后用酶标仪分别测定肝再生过程中不同时期(共8个时间点),肝细胞的凋亡酶活性。结果:Caspase-3活性,与对照组相比,在PH后3、6、72、120和168 h时显著性增高(P<0.001);而整个过程中Caspase-8的活性除了在PH后168 h时高于对照组外(P<0.05),其余时间点没有明显增高。结论:肝再生过程中肝细胞凋亡酶Caspase-3活性的升高可能主要是由内源性线粒体途径引起的。

Objective：To study the changes of hepatic caspase-3 and caspase-8 activities during liver regeneration in rats. Methods： Liver regeneration model was produced by 70% partial hepatectomy （PH） in male SD rats. Then the rats were sacrificed and the liver homogenate were prepared. Hepatic caspase-3 and caspase-8 activities were determined at 8 different time points during the liver regeneration at the absorbance of 405 nm. Results： Hepatic caspase-3 activity increased significantly 3 h, 6,72 h, 120 h and 168 h after PH compared with that of control group（P〈0. 001）; Hepatic caspase-8 activity increased 168 h after PH（P%0.05） and had no obvious change at any other time points. Conclusion： Our result suggests that the increase of caspase-3 activity during the liver regeneration may be mainly induced by the mitochondrial pathway.