燕麦β-葡聚糖治疗大鼠高脂血症

Therapeutic effect of oat β-glucans on serum lipid levels of rats with experimental hyperlipemia

目的:考察燕麦β-葡聚糖对大鼠高脂血症的治疗作用。方法:健康成年SD大鼠70只,雌雄各半,随机分成正常对照组(n=10)和复制模型组(n=60)。复制模型组喂高脂饲料,3周后测定大鼠空腹血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C),以血清TC显著高于正常对照组为造模成功指标。选50只高脂血症大鼠,按血脂随机分为5组(n=10):高脂对照组(等量蒸馏水,灌胃给药),壳寡糖对照组[200 mg/(kg.d),灌胃给药],燕麦β-葡聚糖低、中、高剂量组[133 mg/(kg.d),266 mg/(kg.d),533 mg/(kg.d),均灌胃给药],各组继续喂高脂饲料4周;正常对照组喂普通饲料、蒸馏水4周。在灌胃给药前后分别称每只大鼠体质量。分别于大鼠灌胃给药前及给药3、4周眼眶采血,测定血清TC、HDL-C、LDL-C。结果:与正常对照组相比,燕麦β-葡聚糖高、中剂量组大鼠体质量下降(P<0.05)。燕麦β-葡聚糖能显著降低高脂血症大鼠血清TC和LDL-C,显著升高HDL-C。燕麦β-葡聚糖灌胃4周后降脂作用明显高于灌胃3周后的作用,且呈明显的剂量依赖性;其降脂作用优于壳寡糖,并未见不良反应。结论:燕麦β-葡聚糖对大鼠高脂血症有显著的治疗作用。

Objeetive：To investigate the therapeutic effect of oat β-glucans on serum lipid levels of rats with experimental hyperlipemia. Methods： Rats were fed on high fat forage for 3 weeks to establish hyperlipemia models, then the model rats were orally given oat β-glucans at doses of 133, 266 and 533 mg/（kg · d） separately. At the same time, there were 3 control groups including chitosan ligosaccharide[200 mg/（kg · d）], normal, and high blood lipid （model rats fed with distilled water）. Serum levels of total cholesterol （TC）, high density lipoprotein cholesterol （HDL-C） and low density lipoprotein cholesterol （LDL-C） were assayed in rats of all groups and the results were compared. Results.. β-glucans obviously decreased the levels of TC and LDL-C and increased HDL-C in rat hyperlipemia models. The reduction of serum lipid level in 4-week β-glucans treatment rats was more obvious than that in 3-week β-glucans treatment rats. Besides, β-glucans showed an obvious dosage dependent effect in reducing serum lipid level without adverse effect. Oat β-glucans had a better lipid-reducing effect than ligosaccharide did. Conclusion.. Oat β-glucans can obviously decrease serum lipid in rats with hyperlipemia.