东海药用微生物资源的初步调查研究

Medicinal microorganisms of the East China Sea: a preliminary study

目的:对中国东海药用微生物资源进行初步调查研究。方法:从中国东海采集海水和底泥样品,选用不同的分离培养基,采用无限稀释及平板划线的方法分离出单菌落,利用纸片法和稻瘟霉模型对菌株发酵液进行活性筛选。结果:从样品中分离得到1 041株单菌落,包括506株放线菌,421株细菌和114株真菌,其中具有抗菌活性菌株303株,活性菌株分离率为29.1%。抗稻瘟霉分生孢子活性菌株219株,活性菌株分离率为21.0%。结论:分离得到的抗菌活性菌株,抗稻瘟霉分生孢子活性菌株的比例均达到20.0%以上,说明东海具有丰富的药用微生物资源。

Objective：To investigate the resources of medicinal microorganisms of the East China Sea. Methods： The sea water and sediment samples were collected from the East China Sea. Different culture media were used to isolate the microorganism strains by infinite dilution and streak plate procedure. Then the isolated strains were screened for antimicrobial active strains using paper disc diffusion model and for antimitosis active strains using Pyricularia Oryzae model. Results： A total of 1 041 strains of marine microorganisms were isolated from marine water and sediment samples, including 506 actinomycetes, 114 fungi and 421 bacteria. It was found that 303（29.1 %） strains of marine microorganisms had antimicrobial activity and 219 （21.0%） strains had antimitotic activity. Conclusion.. The proportions of strains with antimicrobial activities and antimitotic activities are both above 20%, suggesting that the East China Sea has rich resources of medicinal microorganisms.