尼美舒利对Alzheimer's大鼠的脑保护作用

Neuroprotective effect of nimesulide on the brain of AD rats

目的:建立大鼠Alzheimer's病(AD)炎症病理模型,探讨非甾体类抗炎药物尼美舒利在AD防治中的作用。方法:25只大鼠随机均分为β淀粉样蛋白(Aβ)组、Aβ+羧甲基纤维素钠组、Aβ+尼美舒利组、假手术组与空白对照组。前3组在大鼠双侧海马CA1区立体定向注射Aβ造AD模型,假手术组注射生理盐水,正常对照组不做任何处理。Aβ+尼美舒利组采用尼美舒利(2.5g/L)灌胃治疗,Aβ+羧甲基纤维素钠组给予50g/L羧甲基纤维素钠。4周后各组大鼠均作Y型迷宫测试观察学习记忆能力。采用免疫组化法检测大鼠脑组织胶质纤维酸性蛋白(GFAP)和Bcl2的表达。结果:与假手术组和空白对照组相比,Aβ组、Aβ+羧甲基纤维素钠组和Aβ+尼美舒利组学习记忆能力显著降低(P<0.05),GFAP及Bcl2阳性细胞数明显增加(P<0.05);与Aβ组及Aβ+羧甲基纤维素钠组相比,Aβ+尼美舒利组学习记忆能力显著改善,GFAP阳性细胞数明显降低,Bcl2阳性细胞数增加(P均<0.05)。结论:在体条件下尼美舒利通过抑制胶质细胞增生及细胞凋亡等机制对AD大鼠发挥脑保护作用。

Aim：To observe the therapeutic effects of nimesulide on Alzheimer＇s disease（AD） rats. Methods：A total of 25 rats were allocated into 5 groups： β-amyloid （Aβ） group, Aβ ＋ sodium carboxymethylcelceulose （Aβ ＋ SC ） group, Aβ ＋ nimesulide group, sham-operation group and normal control group. The first 3 groups were given Aβ to induce AD model, and sham-operation group was given normal saline, then Aβ ＋ SA group and Aβ ＋ nimesulide group were given corresponding drugs, respectively. After 4 weeks the ablity of study and memory was detected by maze test. The expression of GFAP and Bcl-2 was detected using immunochemistry. Results： The nimesulide could ameliorate the ability of study and memory of AD. It could inhibit the hyperplasia of astrocytes and the cell apoptosis induced by Aβ. Conclusion： Nimesulide can improve the damage induced by Aβthrough restraining the hyperplasia of astrocytes and inhibiting the apoptosis of cells in hippocampi.