息痛颗粒对小鼠出凝血时间及血瘀型大鼠血小板聚集的影响

Effects of Xi Tong Ke Li on bleeding time and coagulation time in mice and platelet aggregation in rats

目的:观察息痛颗粒对小鼠出凝血时间及对血瘀型大鼠血小板聚集的影响。方法:小鼠50只,随机分为空白对照组、阳性对照组,息痛颗粒大、中、小剂量组,每组10只,分别以生理盐水,丹参滴丸0.1g/(kg·d),息痛颗粒6.26g/(kg·d)、3.13g/(kg·d)、1.25g/(kg·d)灌胃给药,1次/d,连续3d。于末次灌胃后断尾法测出血时间,用毛细玻管法和玻片法测凝血时间。大鼠60只,随机分成6组,每组10只。血瘀模型组、阳性对照组、息痛颗粒大、中、小剂量组分别以生理盐水,丹参滴丸37.5mg/(kg·d),息痛颗粒5g/(kg·d)、2.5g/(kg·d)、1.25g/(kg·d)灌胃给药,1次/d,连续3d。以上5组均于末次给药后1h用肾上腺素加冷刺激形成大鼠急性“血瘀”模型。以正常大鼠灌服生理盐水作空白对照。第2d各组分别自颈动脉取血,以比浊法测二磷酸腺苷(ADP)、胶原(COL)、花生四烯酸(AA)诱导的血小板最大聚集率。结果:与空白对照组比较,息痛颗粒大、中剂量组小鼠出、凝血时间均延长(P均<0.05);息痛颗粒各组血瘀型大鼠ADP、COL、AA诱导的血小板最大聚集率均降低(P均<0.05)。结论:息痛颗粒有抗血小板聚集的功能。

Aim ：To observe the effects of Xi Tong Ke Li on platelet aggregation in blood-stagnation rats,and bleeding time （BT） and coagulation time（CT） in mice. Methods：Fifty mice were randomly allocated into normal control group, positive control group （ Dan Shen Di Wan ） , and Xi Tong Ke Li low-, middle-, and high-dose groups, 10 mice in each group. BT and CT were determined after 3 days administration; sixty rats were randomly allocated into model group, posi- tive control group, Xi Tong Ke Li low-, middle-, and high-dose groups and normal control group. After 3 days administration, the first 5 groups were established blood-stagnation model by injecting adrenalin and bathing in ice water. The rate of platelet aggregation induced by ADP, COL, AA was determined. Results： Compared with those of the normal control, the platelet aggregation rates of rats in 3 Xi Tong Ke Li groups in high- and middle-dose Xi Tong Ke Li groups were lower （P 〈 0.05 ） ; BT and CT of mice in 3 Xi Tong Ke Li groups were longer （ P 〈 0.05 ）. Conclusion ： Xi Tong Ke Li could inhibit platelet aggregation.