人血清痕量金涂钒石墨管石墨炉原子吸收光谱法测定

Determination of trace gold in human serum by graphite furnace atomic absorption spectrometry with vanadium-coated graphite tube

目的:建立人血清中痕量金的准确、灵敏的测定方法。方法:金与硫代米蚩酮在酸性介质中生成稳定的络合物,用异戊醇萃取后,取有机相直接进样,采用涂钒热解石墨管,石墨炉原子吸收法测定痕量金。结果:该方法的检出限为1.8×10-12g。回收率在92.6%～103.5%。对于10μg/L金的测定表明,该方法的相对标准偏差为2.9%。结论:该方法用于人血清中痕量金的测定结果满意。

Aim ： To develop a new method for the determination of trace gold in human serum. Methods：In the acidic medium, gold and thio-michler＇s ketone（TMK）can form a stable complex. After extracted by isoamylalcohol, the organic phase was injected directly. The trace gold in human serum was determined by graphite furnace atomic absorption spectrometry with the vanadium-coated graphite tube. Results： The detection limit of gold was 1.8 ×10^-12g. The recovery rate was between 92.6% to 103.5%. The standard deviation of 10 μg/L gold was 2.9%. Conclusion： This method can be used to analyze the trace gold in human serum, and the result is satisfactory.