异丙酚对大鼠离体心脏缺血/再灌注损伤时心肌c-fos基因表达的影响

The effect of propofol on c-fos gene expression during global myocardial ischemia-reperfusion in isolated rat heart

目的观察异丙酚对离体大鼠全心缺血再灌注损伤心肌c-fos基因表达的影响,探讨其对缺血再灌注损伤心肌保护作用机制。方法Wistar大鼠36只,随机分为对照组和异丙酚组,每组又分为缺血前、缺血30min和复灌30min3个亚组。通过离体心肌灌注模型,采用免疫组化及RT-PCR观察心肌c-fos蛋白及c-fosmRNA表达水平的变化。结果与缺血前相比,缺血30min及再灌注30min亚组的c-fos蛋白的光密度值及c-fosmRNA表达水平均增加(P<0.01);与对照组相比,异丙酚各亚组的c-fos蛋白光密度值及c-fosmRNA表达水平均降低(P<0.01)。结论c-fos基因参与了心肌缺血再灌注损伤的基因调节。异丙酚可减轻心肌c-fos基因的表达,并可避免或减轻心肌缺血再灌注损伤。

Objective To study the protective effects of propofol on isolated rat heart during global myocardial ischemia-reperfusion. Methods 36 Wistar rats were randomly divided into control group and propofol group. Each group was subdivided into 3 subgroups： preiscbemia, ischemia 30 minutes and ischemia-reperfusion 30 minutes group. The changes in c-fos protein and c-fos mRNA level in isolated Langendorff perfused rat myocardium were assessed by immunohistochemical technique and RT-PCR technique respectively. Resdts Compared to preischemia subgroup the expression of c-fos protein and c-fos mRNA level in ischemia 30 minutes and ischemia-reperfusion 30 minutes subgroups were higher significantly （P〈0. 01）. As compared with the values of control group, the expression of c-fos protein and c-fos mRNA level were lowered in propofol group of iscbemia 30 minutes and reperfusion 30 minutes （P〈0. 01）. Condusion c-fos gene may be involved in molecular modulation of myocardial ischemia-reperfusion injury. Propofol can depress the expression of c-fos gene in myocardium, thus contribute to ameliorate and obviate the myocardial ischemia-reperfusion injury.