不同分化程度大肠癌组织中MAGE-3的表达

Expression of melanoma antigen-encoding 3 (MAGE-3) in the tissues of colorectal cancer (CRC) in different grades of differentiation

目的研究黑色素瘤抗原3(MAGE3)在不同分化程度大肠癌组织中的表达。方法应用免疫组化法对101例大肠癌组织石蜡标本进行MAGE-3抗原表达测定。分析MAGE-3基因产物的表达与大肠癌组织分化及临床分期的关系。结果MAGE-3仅表达于肿瘤组织,阳性率为31.7%,正常组织无一例表达,且低分化大肠癌组织的阳性率及阳性强度均高于高分化大肠癌组织(P<0.05)。MAGE-3在大肠癌组织中的表达与Duckes分期无关(P>0.05)。结论MAGE-3基因有可能作为大肠癌组织分化相关分子标志物,其抗原可能作为大肠癌患者免疫治疗的靶抗原。

Objective To study the expression of MAGE-3 in colorectal cancer （CRC） with differem differemiation grades. Method Immunohistochemical technique for MAGE-3 amtigen was performed in paraffin embedded sections of 101 CRC specimens. The relationship between differemiation and Dukes classification of CRC and the expression of MAGE-3 amigen was analyzed,Result MAGE-3 was only expressed in tumor tissues with the total positive rate of 31.7%. The rate and intensity of MAGE-3 in poorly differentiated group were higher than that of the well differemiated group （P〈0.05）. There was no signifiant relationship between MAGE-3 expression and Dukes stage of CRC （P〈0.05）. Conclusion MAGE-3 may be a specific molecular differemiation related marker of CRC. Its antigen may have potential use as a new peptide vaccine for immunotherapy for CRC.