胍基丁胺对血清诱导大鼠肺动脉平滑肌细胞增殖的影响

EFFECT OF AGMATINE ON PROLIFERATION OF RAT PASMCs INDUCED BY SERUM

目的:观察胍基丁胺对血清诱导大鼠肺动脉血管平滑肌细胞(PASMCs)增殖的影响。方法:采用组织块贴壁法原代培养大鼠PASMCs,取对数生长期PASMCs分对照组和胍基丁胺组,比色法测定细胞培养液中乳酸脱氢酶(LDH)活力,液闪计数仪测定3H-TdR掺入量,流式细胞仪测定细胞周期,图像分析法测定增殖细胞核原含量(PCNA)作为细胞增殖的指标。结果:胍基丁胺对PASMCs培养液中LDH活力无明显影响;胍基丁胺可显著减少血清诱导增殖PASMCs的3H-TdR掺入量和PCNA的含量(P<0.01),使G0/G1期细胞比例显著增加,G2/M期细胞比例显著减少(P<0.01)。随着胍基丁胺浓度的增加,PASMCs3 H-TdR掺入量也相应显著减少(P<0.01)。结论:胍基丁胺对PASMCs不产生明显的细胞毒性作用;但可抑制血清培养大鼠PASMCs的增殖,这种抑制作用呈剂量依赖性。

Aim： To determine the effect of agmatine on the proliferation of PASMCs induced by serum. Methods： Primary culture of rat PASMCs was prepared from adult male Wistar rat pulmonary artery by the method of tissue block anchorage. PASMCs were divided into two groups： control group and agmatine administration group. The activity of LDH in the medium was measured by chromato metry. The ^3H-TdR incorporation was measured by liquid scintillometry. The cell cycle was measured by flow cytometry. The PCNA content was measured by image analysis. Results： Agmatine did not exert significant effect on the activity of LDH in the medium. Agrnatine significantly decreased the ^3H-TdR incorporation and PCNA content of PASMCs. Agrnatine significantly decreased the cell ratio of G2/M phase and increased the cell ratio of G0/G1 phase. With the increment of the concentration of agmatine, ^3H- TdR incorporation was significantly decreased correspondingly. Conclusion： Agmatine has no significant cytotoxic effect on PASMCs. Agrnatine can dose-dependently inhibit the proliferation of rat PASMCs induced by serum.