摘要
The nucleoprotein (N) gene of the coronavirus of pigeon origin (PSH) was cloned and sequenced A pair of primers based on the nucleoprotein gene sequences of group 3 coronaviruses was designed and used to amplify the N gene of PSH The PCR product was purified and cloned into the pMD18-T vector. The positive recombinant pTN was used for sequencing. The cloned 1275bp fragment covered all the open reading frame of N gene and potentially encoded 409 amino acids. The results of comparative sequence analysis indicated that the nucleotide sequence of N gene of this strain shared 67.4%～96.6% identity with group 3 coronaviruses, and the predicted amino acid sequence shared 67.4%～97.8% identity, while only limited identity (<41.5%) was observed with group 1 and group 2 coronaviruses. Based on these results, PCoV PSH strain was identified as a member of group 3 coronaviruses.
The nucleoprotein （N） gene of the coronavirus of pigeon origin （PSH） was cloned and sequenced A pair of primers based on the nucleoprotein gene sequences of group 3 coronaviruses was designed and used to amplify the N gene of PSH The PCR product was purified and cloned into the pMD18-T vector. The positive recombinant pTN was used for sequencing. The cloned 1275bp fragment covered all the open reading frame of N gene and potentially encoded 409 amino acids. The results of comparative sequence analysis indicated that the n.ucleotide sequence of N gene of this strain shared 67.4%～96.6% identity with group 3 coronaviruses, and the predicted amino acid sequence shared 67.4%～97.8% identity, while only limited identity （〈41.5%） was observed with group 1 and group 2 coronaviruses. Based on these results, PCoV PSH strain was identified as a member of group 3 coronaviruses.
出处
《中国病毒学》
CSCD
2006年第3期301-303,共3页
Virologica Sinica
基金
上海交大-昂立抗击"非典"科技基金(SARS03-03)
关键词
鸽
冠状病毒
核衣壳基因
克隆
序列分析
Pigeon
Coronavirus
Nucleoprotein gene
Clone
Sequence analyses
