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骨髓干细胞不同移植途径对急性心肌梗死大鼠心功能的影响 被引量:2

Effects of bone mesenchymal stem cell transplantations via different routes on cardiac function of rats with acute myocardial infarction
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摘要 目的:观察同种异体骨髓间充质干细胞经不同移植途径对急性心肌梗死大鼠心功能的影响,同时评价干细胞移植的有效性和安全性。方法:实验于2004-05/2005-12在哈尔滨医科大学中心实验室完成。①Wistar雄性大鼠50只,采用随机数字表法分成干细胞移植组(n=30)和心肌梗死对照组(n=20)。移植组和对照组结扎左冠状动脉前降支造成急性心肌梗死模型。②冠脉结扎后7d,移植组经股静脉和心外膜分别注入骨髓间充质干细胞(含15×108细胞),对照组注入等量DMEM培养液。③分别于干细胞移植前、移植后1,2和4周行心脏超声检查,术后4周行血液动力学测定,观察心功能的变化。结果:存活43只模型制作成功的大鼠,分为干细胞移植组25只,其中股静脉注射12只,心外膜注射13只;心肌梗死对照组18只。①心肌梗死后4周,移植组左心室射血分数、左心室短轴缩短率、左心室收缩末压和左心室压力最大上升速率比对照组明显提高(左心室射血分数,股静脉:(54.3±1.8)%比(42.4±1.9)%,心外膜:(55.2±1.7)%比(43.5±2.0)%;左心室短轴缩短率,股静脉:(45.0±1.1)%比(33.5±0.9)%,心外膜:(46.5±0.9)%对(32.8±0.7)%;左心室收缩末压,股静脉:(104.2±3.8)比(98.2±4.6)mmHg,心外膜:(105.4±2.3)比(102.3±3.6)mmHg;左心室压力最大上升速率,股静脉:(8290±811)比(6987±612)mmHg/s,心外膜:(8158±745)比(7015±740)mmHg/s;P<0.01)。②左心室收缩末直径、左心室舒张末压、左室压力最大下降速率和左心室等容舒张时间常数均明显减小(P<0.01)。③股静脉移植组和心外膜移植组对心功能的影响差异无显著意义(P>0.05)。结论:经静脉和心外膜两种途径移植骨髓间充质干细胞是安全可行的,二者均能有效地改善心功能,为急性心肌梗死的治疗提供一条行之有效的新途径。 AIM: To observe the effects of allogeneic bone mesenchymal stem cell (BMSC) transplantations via different routes on cardiac function of rats with acute myocardial infarction (AMI), and evaluate the effectiveness and safety of stem cell transplantation (SCT). METHODS: The experiment was conducted at the Central Laboratory of Harbin Medical University between May 2004 and December 2005. ①Fifty male Wistar rats were divided into SCT group (n=30) and AMI control group (n=20) according to random numbers method. AMI was induced by ligations of anterior descending branch of left coronary artery in all the rats. ② Seven days after ligations, BMSCs containing 15×10^8 cells were infused respectively through femoral vein or epicardium of recipient rats in SCT group, while DMEM culture medium of equal dosage was given in the control group. ③ Cardiac function was evaluated before transplantation, 1 week, 2 weeks and 4 weeks after infusion by echocardiography. And hemodynamic measurement was performed at 4 weeks after infusion. RESULTS: The survived 43 rats of successful models were rearranged into SCT group (n=25, including 12 rats of femoral intravenous injection and 13 rats of epicardial injection) and AMI control group (n=18).① Compared with AMI control group, left ventricular ejection fraction (LVEF), fractional shortening (FS), left ventricular end systolic pressure (LVESP) and +dp/dtmax were elevated significantly in SCT group at 4 weeks after AMI [LVEF, femoral vein: (54.3±1.8)%/(42.4±1.9)%, epicardium: (55.2±1.7)%/(43.5±2.0)%; FS, femoral vein: (45.0±1.1)%/(33.5±0.9)%, epicardium: (46.5±0.9)%/(32.8±0.7)%; LVESP, femoral vein: (104.2±3.8)/ (98.2±4.6) mm Hg, epicardium: (105.4±2.3)/(102.3±3.6) mm Hg+dp/dtmax, femoral vein: (8 290±811)/(6 987±612) mm Hg/s, epicardium: (8 158±745)/ (7 015±740) mm Hg/s; P 〈 0.01].②Whereas left ventricular end systolic diameter (LVESD), left ventricular end diastolic pressure (LVEDP), -dp/ dtmax and time constant of left ventricular isovolumic relaxation (Tc) were significantly lower (P 〈 0.01). ③There were no significant difference of cardiac function between femoral intravenous and epicardial transplantation groups(P 〉 0.05). CONCLUSION: Both intravenous and epicardial transplantations of BMSC can effectively improve cardiac function, and provide a feasible, safe and effective way for the treatment of AMI.
出处 《中国临床康复》 CAS CSCD 北大核心 2006年第21期34-36,共3页 Chinese Journal of Clinical Rehabilitation
基金 黑龙江省海外学人资助项目(1053HQ020)~~
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