微重力反应器在细胞培养中的应用(英文)

Application of microgravity bioreactor in cell culture

目的:微重力培养技术是一种新兴的培养技术,可用于组织工程、细胞培养等多个领域。介绍微重力反应器模拟微重力环境用于细胞培养。资料来源:应用计算机检索PubMed1995-01/2002-06的文章,检索词为“microgravity,simulatedmicrogravity,bioreactor,tissueengineering,cellculture,Rotatingcellculturesystem”,限定文章语言种类为English。资料选择:纳入标准:①模拟微重力下细胞培养的研究。②微重力组织工程学的研究。③随机对照实验研究。④微重力环境的创建。排除标准:①较陈旧的文献。②重复研究。资料提炼:共收集到288篇与脊髓损伤修复相关的文献,其中19篇符合纳入标准,排除的269篇文章系同一类重复性研究。资料综合:①模拟微重力培养有其独特的特点和实用性:避免湍流形成的过大剪切力;培养液通过旋转逐渐混合;零顶空间;贴壁依赖性细胞可在载体上生长;提供充分氧合作用。②微重力环境下细胞的生长:细胞易于聚集,产生更高的细胞密度。③微重力反应器可以建立动物细胞的三维培养体系,达到满足器官替代物,或体外研究的要求。④通过微重力反应器所构建的三维组织结构中可产生血管样管状结构,用于产生组织工程学的血管管道。⑤需要解决的问题:微重力反应器所构建的三维结构一旦离开微重力环境,其功能能否长期保持,或移植整合到宿主组织后仍保持不变,尚需进一步研究。结论:模拟微重力培养是一种全新的组织培养技术,其核心技术是建立动物细胞的三维培养体系,可以为多种细胞和组织块的生长和代谢提供良好的培养环境,可以进行高密度的组织培养,并保持所培养细胞的组织分化特异性。

OBJECTIVE： Microgravity culture is an emerging cultural technology, which can be used in many fields such as tissue engineering and cell culture,etc. Microgravity bioreactor was used for cell culture in simulated microgravity environment. DATA SOURCE： We search the PubMed database from January 1995 to June 2002, using the key words ＂microgravity,simulated microgravity, bioreactor, tissue engineering,cell culture, rotating cell culture system＂ and the language was confined to English. STUDY SELECTION： Inclusion criteria： ① Research of cell culture in simulated microgravity condition. ② Research of tissue engineering of microgravity. ③ Randomized controlled empirical study. ④ Establishment of the simulated microgravity environment. Exclusion criteria： ①Quite obsolete literatures. ② Repeated research. DATA EXTRACTION： A total of 288 articles on reparation of spinal cord injury were collected, according to the inclusion criteria 19 articles were included, and the other 269 articles were excluded because they were repetitive and duplicated researches. DATA SYNTHESIS： ①Cultivation in simulated microgravity had its partitular feature and practicability： Sheafing force formed by the turbulent flow was avoided; Culture fluid was admixed by rotating; There exists Zero headspace; Anchorage-dependent cell could grow on the carriers; Suffcient oxygenation was provided. ②Cell growth in simulated microgravity environment： cells were prone to aggregate, and much higher cell density was Three-dimensional system of animal cells was congenerated. ③Three culture structed with microgravity bioreactor to satisfy organ surrogate or requirement of investigation in vitro, ④ Angioid tubiform fabric was generated in the three-dimensional tissue structure constructed with microgravity bioreactor, which was treed to generate the blood vessel pipage of tissue engineering. ⑤ Problems needing to solve： Once separated from the microgravity environment, whether the function of three-dimensional structure constructed with microgravity bioreactor can be still remained for long term or not and the structure will be kept invariably or not after transplanted and integrated to parasitifer tissue need further research. CONCLUSION： Technique of simulated microgravity culture is a original tissue culture technology. The central technique is the construction of three-dimensional culture system of animal cells, which can provide a favourable cultural surrounding for the growth and metabolism of mutiplicate cells and tissue blocks, carry out the tissue culture of high density, and keep the specificity of histological differentiation of cultural ceils.