肾结石患者血清中纳米细菌的分离与培养

Isolation and cultivation of nanobacterium from serum of patients with renal stone

目的分离培养肾结石患者血清中纳米细菌,探讨纳米细菌感染与肾结石发病之间的关系。方法取40例肾结石患者血清,经0．22μm滤膜过滤后,用RPMI 1640细胞培养基于37℃,5％CO2环境中培养,用显微摄影技术记录结果;以40例健康成人血清为对照。结果22例(55％)肾结石患者血清、8例(20％)对照血清中分离到纳米细菌。经X2检验,差异具有显著性(P<0．05)。结论肾结石患者血清中纳米细菌检出率高于正常人血清,提示纳米细菌感染与肾结石发生可能有关。

Objective To isolate and culture nanobacterium from serum of patients with renal stone for study of the relationship betweenrenal stone and nanobacterium infection. Methods Forty serum samples from patients with renal stone were filtered via O. 22 μm membrane,then RPMI1640 was added as nutrient medium. After culture under the condition of 37 ℃ and 5% CO2, micrograph were performed. Other 40 sera from healthy subjects were served as controls. Results The samples of 22 cases （55%） were positive and 8 cases（20%） were positive in controls. There was a significant difference on the infection rate of nanobacterium between patients with renalstone and healthy subjects. Conclusion Since the infection rate of bacterium in patients with renal stone is higher than that in healthysubjects,it may be considered that nanobacterium infection might play a certain role in occurrence of renal stone.