载体介导HER-2RNA干扰治疗乳腺癌研究

Vector-mediated HER-2 RNA interference against HER-2-positive breast cancer

目的探讨载体介导HER-2RNA干扰治疗HER-2阳性乳腺癌的可行性。方法构建能够产生HER-2siRNA的质粒载体,利用载体转染HER-2阳性乳腺癌细胞SKBR-3,利用RT-PCR与Westernblot检测HER-2mRNA与蛋白表达情况,同时观察转染后SKBR-3细胞凋亡及生长情况。结果SKBR-3细胞HER-2受到载体介导的RNA干扰后,HER-2mRNA及蛋白表达出现明显下调,细胞凋亡增加,肿瘤细胞生长受到抑制。结论乳腺癌HER-2是理想的RNA干扰治疗靶点,靶向HER-2的RNA干扰可以诱导肿瘤凋亡,抑制肿瘤生长。

Objective To study the feasibility of vector-mediated RNA interference for HER-2-positive breast cancer therapy. Methods A plasmid vector capable of mediating HER-2 RNA interference was constructed, and HER-2-positive breast cancer cell line SKBR-3 was transfected with this constructed vector. The expression of HER-2 mRNA and protein was analyzed by RT-PCR and Western blotting, and the growth and apoptosis of SKBR-3 cells was analyzed after transfection. Results The expressions of HER-2 mRNA and HER-2 protein was downregulated in response to vector-mediated HER-2 RNA interference, which also resulted in tumor cell growth inhibition and increased number apoptotic cells. Conclusion HER-2 is a good target for RNA interference and RNA interference targeting HER-2 can lead to HER-2 breast cancer cell apoptosis and growth inhibition.