人食管鳞状细胞癌基因表达变化的cDNA芯片研究

cDNA microarray-based study of gene expression profile changes in human esophageal squamous cell carcinoma

目的利用TaKaRa人癌基因芯片研究食管鳞状细胞癌(ESCC)和相应正常组织差异表达基因,分析基因差异表达谱,以期找到更快捷、高通量的方法来筛选与原发性食管癌发生、发展以及与食管癌临床进展相关的分子标记物。方法应用基因芯片技术及激光捕获微切割术、T7RNA聚合酶扩增技术,对15例ESCC癌组织和正常组织的mRNA进行检测,并用生物信息学对检测结果进行分析。结果在886个目的基因中有110条(12.42%)至少2次出现差异表达,其中56条(6.32%)基因表达上调幅度>2.0倍,54条(6.09%)基因表达下调幅度<0.5。结论高通量的基因芯片技术可筛选出大量ESCC相关基因,对这些相关基因功能的验证将有助于找到ESCC发生、发展的关键基因或通路。

Objective To investigate the differentially expressed genes between human esophageal squamous cell carcinoma （ESCC） and normal esophageal mucosa and explore an effective method with high throughput for screening the molecular markers closely correlated with the development, invasion and metastasis of ESCC. Methods With eDNA mieroarray and laser capture microdissection, T7-based amplification were used to detect the mRNA from both the primary carcinoma and the corresponding esophageal epithelium in 15 ESCC cases, and the results were analyzed by bioinformatics methods. Results Among the 886 target genes, 110 （12.42%） genes were differentially expressed commonly at least twice in all the 15 samples, including 56 （6.32%） up-regulated by at least 2 folds and 54 （6.09%） down-regulated by at least 0.5 folds. Conclusion Many ESCC-assoeiated genes were screened by the high-throughput gene chip method, and functional study of these genes may help to identify the key genes or pathways involved in the pathogenesis and development of ESCC.