辣椒叶片离体培养与植株再生

In Vitro Plant Regeneration from Leaf Explants of Pepper(Capsicum annuum L.)

以辣椒无菌苗叶片为外植体，就激素对其不定芽分化及植株再生的影响进行了研究。结果表明，ＢＡ对叶片不定芽的诱导效果最好，ＺＴ次之，ＫＴ不能有效地诱导叶片形成不定芽；ＩＡＡ与ＢＡ相配合使叶片不定芽分化频率明显提高；ＩＢＡ或ＮＡＡ与ＢＡ的激素组合使叶片不定芽分化频率有所不降；２，４－Ｄ对叶片不定芽分化有明显的抑制作用。在分化培养基中添加１．０ｍｇ／ＬＧＡ３有利于不定芽的伸长。不定芽伸长后在不含激素的ＭＳ或ＭＳ＋ＭＡ培养基上诱导生根，形成完整植株。

Effects of hormones on differentiation of adventitious buds and plant re-generation were studied in leaf culture of pepper in vitro.BA was considered as the bestgrowth regulator for adventitious bud differentiation within 9 cytokinins,The combina-tion of IAA and BA raised the differentiation frequency obviously,Thp highest differen-tiation frequency of adventitious buds was obtained in the medium MS+BA6.0mg/L+iAA 1.0mg/L,while the lower ones were observed in the combinations of IBA or NAAwith BA.2,4-D,however,showed the inhlbition effect on the bud differentiation.Adding GA_3 in differentiation media promoted the elongation of adventitious buds,Theelongated shoots rooted in media MS without hormone or MS+IAA,and developed intocomplete plants.