摘要
应用生物化学技术，对香菇单双核杂交菌株进行细胞学分析，以细胞内可溶性蛋白质及酯酶同工酶为指标，对香菇单双核杂交的亲本以及杂交后代进行分析鉴定，认为在所选单双核菌株杂交过程中，单核与双核菌株可能发生了基因重组，单双核杂交技术可作为一种香菇品种改良的方法应用于香菇杂交育种的研究中。
Hybrid shiitake strains resulting from di-mono mating were analyzed by biochemical technology. The parents and hybrids were analyzed and identified by electrophoretogram of esterase iozymes and soluble proteins. The results confirmed that dikaryon and monokaryon had exchanged chromosomes during the di-mono mating. This indicated that dimono mating can be used as a method for Lentinus edodes strain improvement in breeding program.
出处
《上海农业学报》
CSCD
1996年第1期19-22,共4页
Acta Agriculturae Shanghai
关键词
香菇
单双核杂交
育种
同工酶
酯酶
蛋白质
Lentinus edodes
Di-mono mating
Breeding
Esterase isozymes
Protein
