黔产黄褐毛忍冬与国内其他主产区红腺忍冬品质对比研究

Quality comparison of Lonicera fulvotomentosa in Guizhou and Lonicera hypoglauca in other places in China

目的 比较研究贵州黄褐毛忍冬与不同产地红腺忍冬绿原酸含量。方法采用高效液相色谱法，Shim-pack VP-ODS C18色谱柱（150mm×4．6mm，5μm）；流动相为乙腈-0．2％磷酸溶液（13：87）；流速为1．0mL·min^-1。结果本法在0．16-0．96μg·mL^-1呈良好线性关系（r=0．9999），平均回收率为99．09％，RSD为1．73％（n=5）。结论该方法准确可靠、简单快速，可用于黄褐毛忍冬的含量测定。

OBJECTIVE To compare the content of chloragenic acid in Lonicera fulvotomentosa Hsu. et. S. C. Cheng in Guizhou with that in Lonicera hypoglauca Miq in other places. METHODS The contents of chlorogenic acid were determined by HPLC. Shim-pack VP-ODS C18 column was used, phosphate-acetonitrile （13 ： 87） was used as the mo bile phase, and was detected at 327 nm. The flow rate was 1.0 mL · min^-1. RESULTS The linear range of the meth od was 0.16-0. 96 μg · mL^-1 （r=0.999 9）, RSD was 1.73% （n=5）, and the average recovery was 99.09%. CONCLUSIONS The method is simple, fast and accurate for the analysis of Lonicera fulvotomentosa.