摘要
目的 测定正常中国人群红细胞儿茶酚氧位甲基转移酶（COMT）活性浓度值，为研究COMT活性变化在疾病诊断中的应用提供依据。方法采用高效液相色谱法测定279名正常健康人（其中男性134例，女性145例）红细胞中COMT的活性浓度，用SPSS统计软件对性别间测定结果进行检验比较。结果男性和女性红细胞中COMT活性浓度分别为：（16．5±7．4）、（15．5±5．3）nmol·mL RBC^-1·h^-1。活性频率分布男性在6．0～37．4nmol·mL RBC^-1·h^-1，女性为5．4～37．2nmol·mL RBC^-1·h^-1，男女之间COMT活性浓度的差异无统计学意义（P〉0．05）。结论本法灵敏，简便，准确，重复性好，且测定结果与国外一些报道一致，适用于临床应用测定研究。本结果可作为中国健康人红细胞COMT活性正常值及今后研究的重要参考数据。
OBJECTIVE To explore the normal range of catechol-O-methyltransferase （COMT） activity in erythrocyte of Chinese Han people. METHODS To measure the COMT activity in erythrocyte of 279 healthy Chinese Han people （134 males and 145 females） by HPLC, the data were analysed by SPSS. RESULTS The COMT activity in erythrocyte in the male and female was （16.5±7.4） nmol · mL RBC^-1 · h^-1 and （15.5±5.3） nmol · mL RBC^-1· h^-1 respectively, and the distribution of activity in the male was 6.0-37.4 nmol · mL RB^-1· h^-1, and that in the female was 5.4-37.2 nmol · mL RBC^-1 ·h^-1. There was no significant difference between the male and the female （P〉0.05）. CONCLUSIONS The method is sensitive, simple and accurate. The results are identical with the foreign reports, and suitable for clinical measurement and research. The result can be regarded as the normal value of COMT activity in erythrocyte of Chinese healthy Han population and as important referential data in future research.
出处
《中南药学》
CAS
2006年第3期187-189,共3页
Central South Pharmacy
基金
深圳市科技局科技经费资助项目(200104082)
