甲状腺相关眼病患者眼眶脂肪抵抗素的表达

Expression of Resistin in Orbital Adipose Tissue and Adipocyte of Patients with Thyroid Associated Ophthalmopathy

目的通过检测抵抗素在甲状腺相关眼病患者及对照组眼眶脂肪组织和脂肪细胞中的表达,为进一步探讨抵抗素与甲状腺相关眼病的相关性打下基础。方法通过体外原代培养获得前脂肪细胞,前脂肪细胞在分化液的作用下转化为成熟脂肪细胞。用RT—PCR法检测眶脂肪组织、前脂肪细胞及成熟脂肪细胞中抵抗素mRNA的表达,并进行免疫组化检测抵抗素蛋白的表达。结果前脂肪细胞中无抵抗素mRNA及蛋白的表达。成熟脂肪细胞及眶脂肪组织中有抵抗素mRNA及蛋白的表达。免疫组化结果显示抵抗素蛋白位于成熟脂肪细胞胞浆中。结论甲状腺相关眼病患者眶脂肪中抵抗素mRNA及蛋白表达丰富,说明甲状腺相关眼病患者眶脂肪增多是因成熟脂肪细胞所占比例增加、脂肪细胞分化增强所致。

[Objective]To investigate the expression of resistin mRNA and protein in orbital adipose tissues and adipocytes in the patients with and without thyroid associated ophthalmopathy （TAO）. [Methods] The preadipocytes were cultured from the orbital adipose tissues. The preadipoytes stimulated by a differentiation media were differentiated into adipocytes in vitro. The expression of both resistin mRNA and resistin protein in preadipocytes , adipocytes, and adipose tissues were measured by RT-PCR and immunohistochemistry. [Results] There was no expression of resistin mRNA and protein in preadipocytes, but they were expressed in mature adipocytes and adipose tissues. Immunohistochemistry of mature adipocytes and orbital adipose tissues showed that the resistin protein was abundant in adipocyte cytoplasm. [Conclusion] The resistin is an adipocytokine secreted by mature adipocytes. In our results, the expression of resistin mRNA and resistin protein were abundant in the orbital adipose tissue of TAO patients,which implicated that the orbital adipose tissue of TAO patients was mostly consisted of mature adipocytes, the diffentiation of preadipocytes was enhanced.