大鼠供体喉保存的研究

Research of Laryngeal Allograft Preservation

目的研究大鼠喉移植供体喉的保存,探讨喉体不同组织结构对缺血和坏死的耐受情况。方法40只Wister大鼠,采用UW液灌注,低温保存供体鼠喉,分别通过病理光学及透射电镜观察其形态学改变,比较喉不同组织结构的缺血、坏死变化。结果供体喉保存60h后,病理HE染色才能镜检到喉体组织缺血改变,电镜24h即可发现组织细胞的缺血改变,鼠喉各组织结构缺血耐受时间不同,最先出现变化的是粘膜上皮,接着是喉内肌、小涎腺、血管内皮、神经纤维,最后改变的是软骨细胞。结论透射电镜检查能及时地反映喉体组织细胞缺血、坏死情况,UW氏液保存供体喉不宜超过26h。

[Objective] To study preservation of rat donor larynx and discuss ischemic tolerance of different laryngeal tissue. [Methods] Forty Wister rats＇ laryngeal allograft were perfused and preserved by cold UW＇s solution. Laryngeal morphological transform was observed under light microscopy and dectron microscopy. Ischemic change or necrosis of donor rat larynx was compared. [Results] Ischemic ucosal was detected ischemic change at 24 hours under electron microscopy. Ischemic tolerance was difference among different part of rat larynx. Mucosal demonstrates ischemic change firstly, foUowing by muscle, minor salivary gland, vessel, nerve and cartilage finally. [Conclusion] Electron microscopy can be used to detect change of laryngeal allograft early. UW solution was not suitable to preserve larynx more than 26 hours.