摘要
目的:研究Bio-Oss胶原作为骨组织工程支架材料的可行性。方法:抽取成年小型猪骨髓,贴壁法获得骨髓基质细胞(BMSCs),经成骨诱导培养液体外培养、扩增、诱导后观察细胞增殖情况,并进行I型胶原、骨钙素的免疫细胞化学检测。将培养的第3代细胞接种于Bio-Oss胶原,进行超微结构观察,并将Bio-Oss胶原/BMSCs复合物植入裸鼠背部皮下,4、8周后进行组织学检测。结果:当接种密度为0.8×106/ml时,细胞与支架材料之间有最大附着量;VonKossa染色可见钙结节形成;I型胶原、骨钙素免疫细胞化学染色呈阳性;超微结构观察可见细胞生长附着于材料网孔内表面;组织学检测提示,8周时复合物内有新骨形成。结论:Bio-Oss胶原/BMSCs复合物显示成骨活性,Bio-Oss胶原可用作骨组织工程支架材料。
PURPOSE： To study the probability of Bio-Oss collagen used as a scaffold for bone tissue engineering. METHODS： After aspirating bone marrow from the minipig, the bone marrow stroma cells were separated by adherent methods and induced with the conditional medium, the phenotype of the BMSCs was evaluated by immunocytochemistry. The induced yd passage cells and Bio-Oss collagen were mixed to form a complex. After SEM scanning, the complex was implanted into the subcutaneous tissue of the nude mice. Histological observation were taken after 4 and 8 weeks. RESULTS： The growth curve by MTT method showed the most appropriate inoculating concentration was 0.8×10^6/ml.Von Kossa staining proved the formation of mineralization nodules, immunocytochemistry confirmed the expression of collagen I,Osteocalsin; Scanning electron microscopy showed the cells adhered to the inner surface of the Bio-Oss collagen; HE staining showed the new bone formation and vascularization 8 weeks later in the experimental group. CONCLUSION： The complex （Bio-Oss collagen/BMSCs incubated in vitro）showed new bone formation and vascularization in nude mice,the Bio-Oss collagen can be used as a scaffold for bone tissue engineering.
出处
《中国口腔颌面外科杂志》
CAS
2006年第3期215-219,共5页
China Journal of Oral and Maxillofacial Surgery
基金
上海市卫生局重点专科建设基金(05Ⅱ009)
