选择性神经离断咬肌神经动物模型的建立

Establishment of animal model of selectively denervated masseter in rabbits

目的设计及建立选择性失神经咬肌的动物模型,为临床外科提供实验资料和理论依据.方法实验兔子共计105只,选取5只剥离咬肌,仔细解剖咬肌及神经,以明确其走行.再将其余动物随机平均分为4组,1个正常对照组,3个试验组(离断咬肌神经干组、离断咬肌神经上分支组和离断咬肌神经下分支组),每只动物做自身对照,均以右侧咬肌为试验侧,左侧咬肌为对照侧.建立选择性失神经咬肌动物模型,并行超声检测、质量测量,比较肌肉厚度及质量变化.结果术后三组实验测咬肌厚度及质量均显著减小,分别减小至正常的50.77%,75.97%,79.11%及54.01%,75.93%,77.08%.结论本实验解剖分离并选择性切断咬肌神经,首次成功建立了选择性切断咬肌神经的动物模型,离断不同的神经,咬肌的厚度及质量变化也不相同,但不影响实验动物的进食活动.所以,本模型有效、可靠,适用于失神经咬肌的动物实验研究.

AIM： To design and establish the animal model of selectively denervated masseter, in order to provide experimental data and theoretical evidence for clinical surgery. METHODS： In a total of 105 experimental rabbits 5 rabbits were selected for masseter and nerve anatomy, and the others were randomly devided into 4 groups： Normal control group and 3 experimental groups （denervation of masseter nerve stem, of superior division of masseter nerve, and of inferior division of masseter nerve）. In experimental groups, every rabbit served as its own control, that was, the right side was experimental side, and the left side was control side. The animal modle of selectively denervated masseter was established, and the thickness and weight of experimental masseter were compared with the normal＇s by the examination of B type ultrasound and electron equi-armbalance. RESULTS： After the masseterie nerve hod been abseised, the thickness or weight of denervated masseter obviously became thinner or lighter. The thickness and weight of experimental masseter was changed to 50.77% and 54.01% of the normal＇s in group A, to 75.97% and 75.93% in group B , to 79.11% and 77.08% in group C. CONCLUSION： The animal model of selectively denervated masseter had been successfully set up for the first time. The thickness and humid weight of denervated masseter changed differently after different nerves were selected to be abseised. So, the model was available and credible, and was adapted to be used in researching denervated masseter in rabbits.